Traditional non-invasive sample collection has two approaches to try and collect and produce high gDNA recovery. While both methods have benefits, they also have their challenges.
- Self collection, but limited to individuals capable of producing sufficient saliva
- Collection solution contains alcohol
- Yields sheared, short-fragment DNA not optimal for complex downstream applications
- Up to 40% bacterial content
- High gDNA recovery when the full sample is processed (up to 30µg/sample)
- Cold chain processing not needed
Traditional Buccal Swabs
- Low gDNA recovery with high bacterial contamination
- High resampling rate reduces operational efficiency and increases processing costs
- Transit time of dry swabs exponentially accelerates sample degradation
- Swab removal not automation friendly
- Self collection but simple collection
- Subjects compliance 70%-90%
The iSWAB DNA collection device addresses the issue of saliva based collection, where subjects can not produce enough saliva for collection i.e infants and toddlers or people experiencing xerostomia (dry mouth). Since the iSWAB DNA collection device utilizes swabs for collection, it can be much easier to use with subjects that can not produce enough saliva while still recovering as much as or more gDNA than saliva based or traditional buccal swab collection methods.