The iSWAB™ collection system have been designed to address the low DNA recovery and quality (ie. high content of bacterial DNA), cost and processing issues that current noninvasive sample collection approaches suffers from. The innovative design of iSWAB™ collection system allow for removal of the cells captured by the swab and then released into a proprietary lysis and nucleic acid and protein stabilizing buffer. The combination of the mechanistic release of cells and proprietary lysis buffer allows for the collection of high amount of cells in a concentrated manner from single or several swabs in a small amount of lysis buffer while marinating significantly low bacterial contamination. iSWAB™ collection system is designed to facilitate self or assisted swab based sample collection, concentration from a single or multiple swabs into a single tube for room temperature transport and long term storage. In addition the iSWAB™ collection system is optimized for manual processing as well as it can be adapted to common automated sample preparation liquid handling workstations without the need of additional accessories.